Genome Engineering & Stem Cell Center (GESC)

LEADERSHIP: Xiaoxia Cui, PhD

The Genome Engineering and Stem Cell Center (GESC) is a premier fee-for-service Siteman Cancer Center shared resource specializing in genetic engineering primarily using clustered regularly interspaced short palindromic repeats (CRISPR) technologies and reprogramming of primary human samples into induced pluripotent stem cells (iPSCs) as well as differentiation of stem cells into physiologically relevant cell types, such as cardiomyocytes, hepatocytes, or neuronal cultures. GESC also provides molecular biology services including CRISPR screens and comprehensive genotyping. The mission of GESC is to provide Siteman members with access to cost-effective, cutting-edge technologies of gene editing and stem cells. GESC has successfully created over 1,000 gene edited cancer and stem cell lines and more than 500 mouse models, reprogrammed more than 500 patient-derived iPSC lines, and established differentiation protocols in 10 new cell/tissue types. Additionally, innovative new services have been developed, including the use of recombinant adeno-associated virus (rAAV) donors for sequential insertions to achieve multi-kilobase knockins (KI), and KI characterization with LOCK-seq (LOng read sequencing of Captured Kilo-base targets).

LOCATION: 4515 McKinley Building

PRICING: Please contact the core for current pricing of services offered.

TO ACCESS: Xiaoxia Cui at [email protected], 314-362-2906

NIH PUBLIC ACCESS POLICY: As of April 7, 2008, the NIH requires investigators with a publication using Siteman (or other NIH-funded) shared resources to submit (or have submitted for them) their final, peer reviewed manuscripts to PubMed Central(PMC) upon acceptance of publication, to be made publicly available within 12 months of publication. Many journals automatically submit these for authors, but Washington University also has assistance available through the Becker Medical Library. Please see this resource for more information.

PUBLICATION ACKNOWLEDGEMENT: If research supported by the Genome Engineering & Stem Cell Center results in publication, please acknowledge this support by including the following in your publication(s):

We thank the Alvin J. Siteman Cancer Center at Washington University School of Medicine and Barnes-Jewish Hospital in St. Louis, MO., for the use of the Genome Engineering & Stem Cell Center, which provided __________ service. The Siteman Cancer Center is supported in part by an NCI Cancer Center Support Grant #P30 CA091842.